guinea pig anti sp (Neuromics)

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Neuromics guinea pig anti sp
Guinea Pig Anti Sp, supplied by Neuromics, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/guinea+pig+anti+substance+p/Substance+P-Pure/pm41387112-422-10-14
Average 91 stars, based on 11 article reviews

guinea pig anti sp - by Bioz Stars, 2026-09

91/100 stars

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Saline:

Article Title: Activation of acid-sensing ion channel 3 by lysophosphatidylcholine 16:0 mediates psychological stress-induced fibromyalgia-like pain
Article Snippet: .. After three washes with TRIS-buffered saline (TBS), slices were blocked with a TBS solution containing 5% bovine serum albumin and 0.1% Triton X-100 for 2 hr at room temperature, then incubated with primary antibodies at 4 °C overnight [rabbit anti-ATF-3 at 1:1000, Santa Cruz Biotechnology; rabbit anti-phospho-p44/42 Map Kinase (Thr202/Ty204) antibody (pERK) at 1:500, Cell Signaling; goat anti-calcitonin gene-related peptide (CGRP) at 1:500, Bio-Rad; guinea pig anti-substance P at 1:500, Neuromics; mouse anti-Neurofilament 200 kDa Antibody, clone N52, at 1:500, Millipore].Slices were rinsed three times with TBS, then incubated at room temperature for 60 min with DyLight 594-conjugated IB4 (at 1:200, Vector Laboratories) or corresponding AlexaFluor-conjugated secondary antibodies [at 1:200, Alexa 488-conjugated donkey anti-rabbit IgG, Alexa 594-conjugated donkey anti-goat IgG, Alexa 594- conjugated goat anti-guinea pig IgG, and Alexa 594-conjugated goat anti-mouse IgG, Invitrogen]. ..

Incubation:

Article Title: Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents
Article Snippet: The whole brain, lumbar spinal cord, trigeminal ganglia (TGs) and dorsal root ganglia (DRGs) were then sectioned at a thickness of 20 (ganglia) or 30 (brain and spinal cord) microns using either a cryostat (Leica CM1860, Leica Biosystems Inc., Concord, ON, Canada) or a freezing microtome (Leica SM2000R, Leica Biosystems Inc.). .. Sections from lumbar DRGs mounted on gelatin-coated slides were incubated overnight at 4 °C with a 1/500 dilution of rabbit anti-GFP antibody (Millipore cat. AB3080, Etobicoke, ON, Canada or GeneTex cat. GTX30266, Atlanta, GA, USA), 1/1000 of biotin-conjugated isolectin B 4 (IB 4 ; Sigma Aldrich, cat. L-2140, Oakville, ON, Canada), 1/750 guinea pig anti-Substance P (Neuromics, cat. GP14103, Edina, MN, USA) or 1/1000 mouse anti-NF200 (Sigma Aldrich, cat. N0142) in 0.1 M PBS containing 0.3% Triton X100, 3% NGS and 1% BSA. .. Primary antibodies were detected using 1/500 Alexa 488 goat anti-rabbit, 1/1000 streptavidin Alexa 546, 1/500 Alexa 405 goat anti-mouse or 1/500 Alexa goat anti-guinea pig (secondary antibodies were purchased from Life Technologies, Thermo Fisher Scientific, Burlington, ON, Canada).

Labeling:

Article Title: CCR2-targeting pepducins reduce T cell-nociceptor interaction driving bone cancer pain
Article Snippet: .. Sections were labeled with mouse anti-pERK (1:100, Cell Signaling #9101), mouse anti-CD45 (1:500, Bio-Rad #MCA43R), mouse anti-CD68 (1:500, Bio-Rad #MCA341R), mouse anti-CD11b (1:500, Millipore, #CBL1512), and mouse anti-NeuN (1:500, Millipore #MAB377), rabbit anti-CCL2 (1:100, PeproTech #500-P76), rabbit anti-connexin 43 (1:500, Sigma #6219), rabbit anti-GFAP (1:500, Sigma, #G3893), rabbit anti-Iba1 (1:500, Wako #019–19741), rabbit anti-pP38 (1:100, Cell Signaling, #4511), rabbit anti-Ki67 (1:100, Abcam #AB16667), and mouse anti-pJNK (1:100, Cell Signaling #9251), chicken anti-CCR2 (1:200, Aves Labs, custom) and chicken anti-CGRP (1:500, Millipore #AB5705), guinea-pig anti-substance P (1:500, Neuromics #GP14110), hamster anti-CD3 (1:500, Santa Cruz, #sc-1174) in blocking buffer for three days at 37°C for spinal cord, 24 hour at R.T. for DRG. .. They were further incubated with fluorophore-conjugated secondary antibodies (1:500, AlexaFluor 488, 568, 647, Invitrogen) and spinal cord sections were mounted on SuperFrost Plus slides.

Article Title: Discovery of a CCR2-targeting pepducin therapy for chronic pain.
Article Snippet: .. Sections were labeled with mouse antipERK (1:100, Cell Signaling #9101), mouse anti-CD45 (1:500, BioRad #MCA43R), mouse anti-CD68 (1:500, Bio-Rad #MCA341R), mouse anti-CD11b (1:500, Millipore, #CBL1512), and mouse anti-NeuN (1:500, Millipore #MAB377), rabbit anti-CCL2 (1:100, PeproTech #500-P76), rabbit anti-connexin 43 (1:500, Sigma #6219), rabbit antiGFAP (1:500, Sigma, #G3893), rabbit anti-Iba1 (1:500, Wako #019–19741), rabbit anti-pP38 (1:100, Cell Signaling, #4511), rabbit anti-Ki67 (1:100, Abcam #AB16667), and mouse anti-pJNK (1:100, Cell Signaling #9251), chicken anti-CCR2 (1:200, Aves Labs, custom) and chicken anti-CGRP (1:500, Millipore #AB5705), guinea-pig anti-substance P (1:500, Neuromics #GP14110), hamster anti-CD3 (1:500, Santa Cruz, #sc-1174) in blocking buffer for three days at 37◦C for spinal cord, 24 hour at R.T. for DRG. .. They were further incubated with fluorophoreconjugated secondary antibodies (1:500, AlexaFluor 488, 568, 647, Invitrogen) and spinal cord sections were mounted on SuperFrost Plus slides.

Blocking Assay:

Next-Generation Sequencing:

other:

Article Title: Gastrin-Releasing Peptide-Expressing Nerves Comprise Subsets of Human Cutaneous Aδ and C Fibers that May Sense Pruritus
Article Snippet: Primary antibodies were as follows: rabbit anti-gastrin releasing peptide (GRP, 1:2750; Biomol, now Enzolifesciences, Farmingdale, NY), guinea pig anti-substance P (SP, 1:900; Neuromics, Edina, MN), mouse anti-calcitonin-gene-related peptide (CGRP, 1:250; Enzo Life Sciences), mouse anti-protein gene product 9.5 (1:18; Abcam, Cambridge, MA), guinea pig anti-protein gene product 9.5 (PGP9.5, 1:200, Neuromics), chicken anti-neurofilament 200 (1:20,000; Pierce, Rockford, IL).

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